Fusarium ear rot and how to screen for resistance in open pollinated maize in the Andean regions

Ears infected with ear rot were collected from five provinces in Ecuador. Of the 44 samples analysed 26 carried Fusarium verticillioides, 11 F. subglutinans, two F. graminearum and five carried fungi different from Fusarium. The pathogenicity of ten isolates, seven of F. verticillioides and three of...

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Autores Principales: Silva, Edison, Mora C., Eloy, Medina, A., Vásquez G., José, Valdez, D., Danial, Daniel L., Parlevliet, J. E.
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Publicado: 2018
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Acceso en línea:http://repositorio.iniap.gob.ec/handle/41000/4818
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spelling ir-:41000-48182018-11-07T16:59:42Z Fusarium ear rot and how to screen for resistance in open pollinated maize in the Andean regions Euphytica 153(3):329–337 Silva, Edison Mora C., Eloy Medina, A. Vásquez G., José Valdez, D. Danial, Daniel L. Parlevliet, J. E. MAÍZ ZEA MAYS PODREDUMBRE FUSARIUM MÉTODOS DE INOCULACIÓN PATOGÉNESIS RESISTENCIA TENSIÓN Ears infected with ear rot were collected from five provinces in Ecuador. Of the 44 samples analysed 26 carried Fusarium verticillioides, 11 F. subglutinans, two F. graminearum and five carried fungi different from Fusarium. The pathogenicity of ten isolates, seven of F. verticillioides and three of F. subglutinans, were tested. Per isolate 30 ears of the susceptible cultivar Mishca were inoculated by pricking a steel pin, dipped into a spore suspension, through the husks in the central part of the ear 14 days after mid-silk. Ears inoculated with sterile water and ears without any treatment, natural infection, served as controls. The disease severity (DS) of the ears ranged from 14 to 58% ear rot, the range being similar for both species. The DS of the water control, 19%, was much higher than that of the natural control of 2%. Five strains gave a DS of over 40%, significantly higher than the water control. The DS of the others were similar to the water control. In a series of experiments the effect of various methods of applying Fusarium spores through the husks into young ears were compared. All tested methods resulted in DSs significantly higher than those of the two controls. Inoculation with tooth picks and steel pins dipped in a spore suspension gave similar ear rot percentages. Inoculations at 7 to 14 days after mid-silk produced the highest DS’s. There was no significant effect of spore concentration on the DS. Cultivars differed considerably, the range being from around 20% to over 50%. Surprisingly, only wounding the husks, the sterile water control, resulted in a fairly high DS, much higher than that of the natural control. As the ranking order of the cultivars after wounding only and after inoculation did not seem to be different from the ranking order of the natural control it is suggested to use in areas with high inoculum pressures like the Andes only wounding by means of a steel pin for screening for resistance to maize ear rot. 2018-02-15T15:35:37Z 2018-02-15T15:35:37Z 2007-02 Revista Artículo *EC-INIAP-BEESC-MGC. Quito (Euphytica 153(3):329–337) http://repositorio.iniap.gob.ec/handle/41000/4818 en p. 329–337 application/pdf E. E. Santa Catalina
institution INIAP
collection Repositorio INIAP
biblioteca Biblioteca INIAP
language en
format Artículos
topic MAÍZ
ZEA MAYS
PODREDUMBRE
FUSARIUM
MÉTODOS DE INOCULACIÓN
PATOGÉNESIS
RESISTENCIA
TENSIÓN
spellingShingle MAÍZ
ZEA MAYS
PODREDUMBRE
FUSARIUM
MÉTODOS DE INOCULACIÓN
PATOGÉNESIS
RESISTENCIA
TENSIÓN
Silva, Edison
Mora C., Eloy
Medina, A.
Vásquez G., José
Valdez, D.
Danial, Daniel L.
Parlevliet, J. E.
Fusarium ear rot and how to screen for resistance in open pollinated maize in the Andean regions
description Ears infected with ear rot were collected from five provinces in Ecuador. Of the 44 samples analysed 26 carried Fusarium verticillioides, 11 F. subglutinans, two F. graminearum and five carried fungi different from Fusarium. The pathogenicity of ten isolates, seven of F. verticillioides and three of F. subglutinans, were tested. Per isolate 30 ears of the susceptible cultivar Mishca were inoculated by pricking a steel pin, dipped into a spore suspension, through the husks in the central part of the ear 14 days after mid-silk. Ears inoculated with sterile water and ears without any treatment, natural infection, served as controls. The disease severity (DS) of the ears ranged from 14 to 58% ear rot, the range being similar for both species. The DS of the water control, 19%, was much higher than that of the natural control of 2%. Five strains gave a DS of over 40%, significantly higher than the water control. The DS of the others were similar to the water control. In a series of experiments the effect of various methods of applying Fusarium spores through the husks into young ears were compared. All tested methods resulted in DSs significantly higher than those of the two controls. Inoculation with tooth picks and steel pins dipped in a spore suspension gave similar ear rot percentages. Inoculations at 7 to 14 days after mid-silk produced the highest DS’s. There was no significant effect of spore concentration on the DS. Cultivars differed considerably, the range being from around 20% to over 50%. Surprisingly, only wounding the husks, the sterile water control, resulted in a fairly high DS, much higher than that of the natural control. As the ranking order of the cultivars after wounding only and after inoculation did not seem to be different from the ranking order of the natural control it is suggested to use in areas with high inoculum pressures like the Andes only wounding by means of a steel pin for screening for resistance to maize ear rot.
author Silva, Edison
Mora C., Eloy
Medina, A.
Vásquez G., José
Valdez, D.
Danial, Daniel L.
Parlevliet, J. E.
author_facet Silva, Edison
Mora C., Eloy
Medina, A.
Vásquez G., José
Valdez, D.
Danial, Daniel L.
Parlevliet, J. E.
author_sort Silva, Edison
title Fusarium ear rot and how to screen for resistance in open pollinated maize in the Andean regions
title_short Fusarium ear rot and how to screen for resistance in open pollinated maize in the Andean regions
title_full Fusarium ear rot and how to screen for resistance in open pollinated maize in the Andean regions
title_fullStr Fusarium ear rot and how to screen for resistance in open pollinated maize in the Andean regions
title_full_unstemmed Fusarium ear rot and how to screen for resistance in open pollinated maize in the Andean regions
title_sort fusarium ear rot and how to screen for resistance in open pollinated maize in the andean regions
publishDate 2018
url http://repositorio.iniap.gob.ec/handle/41000/4818
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score 11,871979