Vista Equipo: Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves

Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves

Quinoa (Chenopodium quinoa Willd.) is a highly nutritious pseudocereal with an outstanding protein, vitamin, mineral and nutraceutical content. The leaves, flowers and seed coat of quinoa contain triterpenoid saponins, which impart bitterness to the grain and make them unpalatable without postharves...

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Autores Principales:	Fiallos-Jurado, Jennifer, Pollier, Jacob, Moses, Tessa, Arendt, Philipp, Barriga-Medina, Noelia, Morillo, Eduardo, Arahana, Venancio, Torres, Maria de Lourdes, Goossens, Alain, Leon-Reyes, Antonio
Formato:	Artículos
Lenguaje:	en
Publicado:	2018
Materias:	QUINUA CHENOPODIUM QUINOA SAPONINAS CULTIVOS ANDINOS
Acceso en línea:	http://repositorio.iniap.gob.ec/handle/41000/4838
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spelling	ir-:41000-48382018-04-11T16:21:47Z Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves Plant Science 250:188–197 Fiallos-Jurado, Jennifer Pollier, Jacob Moses, Tessa Arendt, Philipp Barriga-Medina, Noelia Morillo, Eduardo Arahana, Venancio Torres, Maria de Lourdes Goossens, Alain Leon-Reyes, Antonio QUINUA CHENOPODIUM QUINOA SAPONINAS CULTIVOS ANDINOS Quinoa (Chenopodium quinoa Willd.) is a highly nutritious pseudocereal with an outstanding protein, vitamin, mineral and nutraceutical content. The leaves, flowers and seed coat of quinoa contain triterpenoid saponins, which impart bitterness to the grain and make them unpalatable without postharvest removal of the saponins. In this study, we quantified saponin content in quinoa leaves from Ecuadorian sweet and bitter genotypes and assessed the expression of saponin biosynthetic genes in leaf samples elicited with methyl jasmonate. We found saponin accumulation in leaves after MeJA treatment in both ecotypes tested. As no reference genes were available to perform qPCR in quinoa, we mined publicly available RNA-Seq data for orthologs of 22 genes known to be stably expressed in Arabidopsis thaliana using geNorm, NormFinder and BestKeeper algorithms. The quinoa ortholog of At2g28390 (Monensin Sensitivity 1, MON1) was stably expressed and chosen as a suitable reference gene for qPCR analysis. Candidate saponin biosynthesis genes were screened in the quinoa RNA-Seq data and subsequent functional characterization in yeast led to the identification of CqbAS1, CqCYP716A78 and CqCYP716A79. These genes were found to be induced by MeJA, suggesting this phytohormone might also modulate saponin biosynthesis in quinoa leaves. Knowledge of the saponin biosynthesis and its regulation in quinoa may aid the further development of sweet cultivars that do not require postharvest processing. 2018-02-23T20:39:05Z 2018-02-23T20:39:05Z 2016-09 Revista Artículo *EC-INIAP-BEESC-MGC. Quito (Plant Science 250:188–197) http://repositorio.iniap.gob.ec/handle/41000/4838 en p. 188–197 application/pdf E. E. Santa Catalina
institution	INIAP
collection	Repositorio INIAP
biblioteca	Biblioteca INIAP
language	en
format	Artículos
topic	QUINUA CHENOPODIUM QUINOA SAPONINAS CULTIVOS ANDINOS
spellingShingle	QUINUA CHENOPODIUM QUINOA SAPONINAS CULTIVOS ANDINOS Fiallos-Jurado, Jennifer Pollier, Jacob Moses, Tessa Arendt, Philipp Barriga-Medina, Noelia Morillo, Eduardo Arahana, Venancio Torres, Maria de Lourdes Goossens, Alain Leon-Reyes, Antonio Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves
description	Quinoa (Chenopodium quinoa Willd.) is a highly nutritious pseudocereal with an outstanding protein, vitamin, mineral and nutraceutical content. The leaves, flowers and seed coat of quinoa contain triterpenoid saponins, which impart bitterness to the grain and make them unpalatable without postharvest removal of the saponins. In this study, we quantified saponin content in quinoa leaves from Ecuadorian sweet and bitter genotypes and assessed the expression of saponin biosynthetic genes in leaf samples elicited with methyl jasmonate. We found saponin accumulation in leaves after MeJA treatment in both ecotypes tested. As no reference genes were available to perform qPCR in quinoa, we mined publicly available RNA-Seq data for orthologs of 22 genes known to be stably expressed in Arabidopsis thaliana using geNorm, NormFinder and BestKeeper algorithms. The quinoa ortholog of At2g28390 (Monensin Sensitivity 1, MON1) was stably expressed and chosen as a suitable reference gene for qPCR analysis. Candidate saponin biosynthesis genes were screened in the quinoa RNA-Seq data and subsequent functional characterization in yeast led to the identification of CqbAS1, CqCYP716A78 and CqCYP716A79. These genes were found to be induced by MeJA, suggesting this phytohormone might also modulate saponin biosynthesis in quinoa leaves. Knowledge of the saponin biosynthesis and its regulation in quinoa may aid the further development of sweet cultivars that do not require postharvest processing.
author	Fiallos-Jurado, Jennifer Pollier, Jacob Moses, Tessa Arendt, Philipp Barriga-Medina, Noelia Morillo, Eduardo Arahana, Venancio Torres, Maria de Lourdes Goossens, Alain Leon-Reyes, Antonio
author_facet	Fiallos-Jurado, Jennifer Pollier, Jacob Moses, Tessa Arendt, Philipp Barriga-Medina, Noelia Morillo, Eduardo Arahana, Venancio Torres, Maria de Lourdes Goossens, Alain Leon-Reyes, Antonio
author_sort	Fiallos-Jurado, Jennifer
title	Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves
title_short	Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves
title_full	Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves
title_fullStr	Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves
title_full_unstemmed	Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves
title_sort	saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in chenopodium quinoa leaves
publishDate	2018
url	http://repositorio.iniap.gob.ec/handle/41000/4838
_version_	1635512300489146368
score	11,871979

Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves

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