Aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de Vernonanthura patens (Kunth) H. Rob. con actividad antif?ngica.
Introduction: the Ecuadorian folk medicine uses the coction from Vernonanthura pathens (Rob H. Kunth) leaves to treat, malaria, pain before and after childbirth, stomachache, skin rashes, diarrheas and parasitism. Objectives: to examine the presence of antifungal activity in fractions obtained fro...
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Formato: | Artículos |
Lenguaje: | spa |
Publicado: |
2017
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Acceso en línea: | http://repositorio.educacionsuperior.gob.ec/handle/28000/4001 |
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Sumario: | Introduction: the Ecuadorian folk medicine uses the coction from Vernonanthura pathens (Rob H. Kunth) leaves to treat, malaria, pain before and after childbirth, stomachache, skin rashes, diarrheas and parasitism.
Objectives: to examine the presence of antifungal activity in fractions obtained from a methanol extract from V. patens leaves.
Methods: the methanol extract from the leaves was fractionated by column chromatography using the following solvent systems: hexane, hexane/ethyl acetate, ethyl acetate and ethyl acetate/methanol. Antifungal activity was measured by the agar diffusion technique with PDA medium in 5 mm wells, by adding 20 ?L fractions at concentrations of 100 and 200 ?g/mL dissolved in dimethylsulfoxide. The active fraction was analyzed for structural identification by gas chromatography-mass spectrometry.
Results: of the fractions obtained, only the 100% hexane fraction showed antifungal activity, since its inhibitory percentages were 57.6 and 80.2 % against Penicillium notatum and 64.8 and 81.5 % vs. Fusaryum oxysporum on the fifteenth day of incubation. The structures of 29 compounds were suggested to be the constituents of the hexane fraction, most of them hydrocarbons.
Conclusions: the presence of antifungal activity in the 100 % hexane fraction was proved, which can extend the medicinal uses of the studied plant and to enrich the Ecuadorian folk medicine. |
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