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Aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de Vernonanthura patens (Kunth) H. Rob. con actividad antif?ngica.

Introduction: the Ecuadorian folk medicine uses the coction from Vernonanthura pathens (Rob H. Kunth) leaves to treat, malaria, pain before and after childbirth, stomachache, skin rashes, diarrheas and parasitism. Objectives: to examine the presence of antifungal activity in fractions obtained fro...

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Autor Principal: Miranda Mart?nez, Migdalia
Formato: Artículos
Lenguaje:spa
Publicado: 2017
Materias:
VERNONANTHURA PATENS
LARITACO
PENICILLIUM NOTATUM
FUSARIUM OXYSPORUM
ANTIF?NGICO
Acceso en línea:http://repositorio.educacionsuperior.gob.ec/handle/28000/4001
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spelling oai:localhost:28000-40012017-04-13T18:00:32Z Aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de Vernonanthura patens (Kunth) H. Rob. con actividad antif?ngica. Miranda Mart?nez, Migdalia VERNONANTHURA PATENS LARITACO PENICILLIUM NOTATUM FUSARIUM OXYSPORUM ANTIF?NGICO Introduction: the Ecuadorian folk medicine uses the coction from Vernonanthura pathens (Rob H. Kunth) leaves to treat, malaria, pain before and after childbirth, stomachache, skin rashes, diarrheas and parasitism. Objectives: to examine the presence of antifungal activity in fractions obtained from a methanol extract from V. patens leaves. Methods: the methanol extract from the leaves was fractionated by column chromatography using the following solvent systems: hexane, hexane/ethyl acetate, ethyl acetate and ethyl acetate/methanol. Antifungal activity was measured by the agar diffusion technique with PDA medium in 5 mm wells, by adding 20 ?L fractions at concentrations of 100 and 200 ?g/mL dissolved in dimethylsulfoxide. The active fraction was analyzed for structural identification by gas chromatography-mass spectrometry. Results: of the fractions obtained, only the 100% hexane fraction showed antifungal activity, since its inhibitory percentages were 57.6 and 80.2 % against Penicillium notatum and 64.8 and 81.5 % vs. Fusaryum oxysporum on the fifteenth day of incubation. The structures of 29 compounds were suggested to be the constituents of the hexane fraction, most of them hydrocarbons. Conclusions: the presence of antifungal activity in the 100 % hexane fraction was proved, which can extend the medicinal uses of the studied plant and to enrich the Ecuadorian folk medicine. http://scielo.sld.cu/pdf/far/v46n3/far08312.pdf 2017-04-12T18:19:39Z 2017-04-12T18:19:39Z 2012 article Manzano Santana, Patricia. et al. (2012). Aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de Vernonanthura patens (Kunth) H. Rob. con actividad antif?ngica. Revista Cubana de Farmacia. Vol. 46(3). Cuba. pp. 352-358 http://repositorio.educacionsuperior.gob.ec/handle/28000/4001 spa openAccess http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ pp. 352-358
institution SENESCYT
collection Repositorio SENESCYT
biblioteca Biblioteca Senescyt
language spa
format Artículos
topic VERNONANTHURA PATENS
LARITACO
PENICILLIUM NOTATUM
FUSARIUM OXYSPORUM
ANTIF?NGICO
spellingShingle VERNONANTHURA PATENS
LARITACO
PENICILLIUM NOTATUM
FUSARIUM OXYSPORUM
ANTIF?NGICO
Miranda Mart?nez, Migdalia
Aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de Vernonanthura patens (Kunth) H. Rob. con actividad antif?ngica.
description Introduction: the Ecuadorian folk medicine uses the coction from Vernonanthura pathens (Rob H. Kunth) leaves to treat, malaria, pain before and after childbirth, stomachache, skin rashes, diarrheas and parasitism. Objectives: to examine the presence of antifungal activity in fractions obtained from a methanol extract from V. patens leaves. Methods: the methanol extract from the leaves was fractionated by column chromatography using the following solvent systems: hexane, hexane/ethyl acetate, ethyl acetate and ethyl acetate/methanol. Antifungal activity was measured by the agar diffusion technique with PDA medium in 5 mm wells, by adding 20 ?L fractions at concentrations of 100 and 200 ?g/mL dissolved in dimethylsulfoxide. The active fraction was analyzed for structural identification by gas chromatography-mass spectrometry. Results: of the fractions obtained, only the 100% hexane fraction showed antifungal activity, since its inhibitory percentages were 57.6 and 80.2 % against Penicillium notatum and 64.8 and 81.5 % vs. Fusaryum oxysporum on the fifteenth day of incubation. The structures of 29 compounds were suggested to be the constituents of the hexane fraction, most of them hydrocarbons. Conclusions: the presence of antifungal activity in the 100 % hexane fraction was proved, which can extend the medicinal uses of the studied plant and to enrich the Ecuadorian folk medicine.
author Miranda Mart?nez, Migdalia
author_facet Miranda Mart?nez, Migdalia
author_sort Miranda Mart?nez, Migdalia
title Aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de Vernonanthura patens (Kunth) H. Rob. con actividad antif?ngica.
title_short Aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de Vernonanthura patens (Kunth) H. Rob. con actividad antif?ngica.
title_full Aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de Vernonanthura patens (Kunth) H. Rob. con actividad antif?ngica.
title_fullStr Aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de Vernonanthura patens (Kunth) H. Rob. con actividad antif?ngica.
title_full_unstemmed Aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de Vernonanthura patens (Kunth) H. Rob. con actividad antif?ngica.
title_sort aislamiento y caracterizaci?n de la fracci?n hex?nica de las hojas de vernonanthura patens (kunth) h. rob. con actividad antif?ngica.
publishDate 2017
url http://repositorio.educacionsuperior.gob.ec/handle/28000/4001
_version_ 1634995211033640960
score 11,871979

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