In vitro anti-protozoal assessment of Vernonanthura patents extracts.

In this work, it was performed a chemical-biological study of Vernonanthura patens (Kunth) H. Rob.?s aerial parts, which grows on the Ecuador?s coast. This work begins with the obtaining of extracts, the isolation and purification of leaves and stems?s fractions and compounds of the species by extra...

Full description

Saved in:
Bibliographic Details
Main Author: Miranda Mart?nez, Migdalia
Format: Artículos
Language:eng
Published: 2017
Subjects:
Online Access:http://repositorio.educacionsuperior.gob.ec/handle/28000/4055
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this work, it was performed a chemical-biological study of Vernonanthura patens (Kunth) H. Rob.?s aerial parts, which grows on the Ecuador?s coast. This work begins with the obtaining of extracts, the isolation and purification of leaves and stems?s fractions and compounds of the species by extraction with ethanol by maceration, chromatography column with increasing polarity?s solvents and TLC. Structural identification was performed by Gaseous chromatography - mass spectrometry and NMR. Anti-protozoal activity was evaluated against Leishmania amazonensis MHOM/77BR/LTB0016?s and Plasmodium berghei ANKA. The majority chemical compounds identified by GC-MS with more than 50% relative abundance were: hexadecanoic acid (palmitic), tetracosanoic acid (lignoceric), tetradecanoic acid (myristic), aristolona, ?-Amyrin acetate, lupeol acetate, lupeol, Lupeol palmitate, stigmasta-5,22- dien-3-ol. Leave?s ethanol extract showed antileishmanial?s activity and selectivity with IC50 of 24, 3 ?g/mL and IS 12, higher than Pentamidine?s positive control (IS 9) such as reported for species that grows in other countries. Investigation?s results are reported forthe first time forthe ecuatorian species.