In vitro anti-protozoal assessment of Vernonanthura patents extracts.

In this work, it was performed a chemical-biological study of Vernonanthura patens (Kunth) H. Rob.?s aerial parts, which grows on the Ecuador?s coast. This work begins with the obtaining of extracts, the isolation and purification of leaves and stems?s fractions and compounds of the species by extra...

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Main Author: Miranda Mart?nez, Migdalia
Format: Artículos
Language:eng
Published: 2017
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Online Access:http://repositorio.educacionsuperior.gob.ec/handle/28000/4055
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spelling oai:localhost:28000-40552017-04-13T18:08:49Z In vitro anti-protozoal assessment of Vernonanthura patents extracts. Miranda Mart?nez, Migdalia VERNONANTHURA PATENS ANTI-PROTOZOAL ACTIVITY LEISHMANIA AMAZONENSIS PLASMODIUM BERGHEI In this work, it was performed a chemical-biological study of Vernonanthura patens (Kunth) H. Rob.?s aerial parts, which grows on the Ecuador?s coast. This work begins with the obtaining of extracts, the isolation and purification of leaves and stems?s fractions and compounds of the species by extraction with ethanol by maceration, chromatography column with increasing polarity?s solvents and TLC. Structural identification was performed by Gaseous chromatography - mass spectrometry and NMR. Anti-protozoal activity was evaluated against Leishmania amazonensis MHOM/77BR/LTB0016?s and Plasmodium berghei ANKA. The majority chemical compounds identified by GC-MS with more than 50% relative abundance were: hexadecanoic acid (palmitic), tetracosanoic acid (lignoceric), tetradecanoic acid (myristic), aristolona, ?-Amyrin acetate, lupeol acetate, lupeol, Lupeol palmitate, stigmasta-5,22- dien-3-ol. Leave?s ethanol extract showed antileishmanial?s activity and selectivity with IC50 of 24, 3 ?g/mL and IS 12, higher than Pentamidine?s positive control (IS 9) such as reported for species that grows in other countries. Investigation?s results are reported forthe first time forthe ecuatorian species. http://pharmacologyonline.silae.it/files/archives/2014/vol1/PhOL_2014_1_A01_Manzano.pdf 2017-04-12T18:56:38Z 2017-04-12T18:56:38Z 2014 article Manzano Santana, Patricia et al. (2014). In vitro anti-protozoal assessment of Vernonanthura patents extracts. Pharmacologyonline. Vol 1. Estados Unidos. pp. 1-6 1827-8620 http://repositorio.educacionsuperior.gob.ec/handle/28000/4055 eng openAccess http://creativecommons.org/licenses/by-nc-sa/3.0/ec/ pp. 1-6
institution SENESCYT
collection Repositorio SENESCYT
biblioteca Biblioteca Senescyt
language eng
format Artículos
topic VERNONANTHURA PATENS
ANTI-PROTOZOAL ACTIVITY
LEISHMANIA AMAZONENSIS
PLASMODIUM BERGHEI
spellingShingle VERNONANTHURA PATENS
ANTI-PROTOZOAL ACTIVITY
LEISHMANIA AMAZONENSIS
PLASMODIUM BERGHEI
Miranda Mart?nez, Migdalia
In vitro anti-protozoal assessment of Vernonanthura patents extracts.
description In this work, it was performed a chemical-biological study of Vernonanthura patens (Kunth) H. Rob.?s aerial parts, which grows on the Ecuador?s coast. This work begins with the obtaining of extracts, the isolation and purification of leaves and stems?s fractions and compounds of the species by extraction with ethanol by maceration, chromatography column with increasing polarity?s solvents and TLC. Structural identification was performed by Gaseous chromatography - mass spectrometry and NMR. Anti-protozoal activity was evaluated against Leishmania amazonensis MHOM/77BR/LTB0016?s and Plasmodium berghei ANKA. The majority chemical compounds identified by GC-MS with more than 50% relative abundance were: hexadecanoic acid (palmitic), tetracosanoic acid (lignoceric), tetradecanoic acid (myristic), aristolona, ?-Amyrin acetate, lupeol acetate, lupeol, Lupeol palmitate, stigmasta-5,22- dien-3-ol. Leave?s ethanol extract showed antileishmanial?s activity and selectivity with IC50 of 24, 3 ?g/mL and IS 12, higher than Pentamidine?s positive control (IS 9) such as reported for species that grows in other countries. Investigation?s results are reported forthe first time forthe ecuatorian species.
author Miranda Mart?nez, Migdalia
author_facet Miranda Mart?nez, Migdalia
author_sort Miranda Mart?nez, Migdalia
title In vitro anti-protozoal assessment of Vernonanthura patents extracts.
title_short In vitro anti-protozoal assessment of Vernonanthura patents extracts.
title_full In vitro anti-protozoal assessment of Vernonanthura patents extracts.
title_fullStr In vitro anti-protozoal assessment of Vernonanthura patents extracts.
title_full_unstemmed In vitro anti-protozoal assessment of Vernonanthura patents extracts.
title_sort in vitro anti-protozoal assessment of vernonanthura patents extracts.
publishDate 2017
url http://repositorio.educacionsuperior.gob.ec/handle/28000/4055
_version_ 1634995216028008448
score 11,871979